English: (A) Continous blistered skin (yellow arrows) and crusts (black arrows) from epidermolysis bullosa patient deficient in the β3 isoform of Laminin5 (LAMB3) before graft. (B) Ex vivo genetic correction of LAMB3 in keratinocyte culture after transduction with retrovirus expressing LAMB3. Scale bars, 100 μm. (C) LAMB3 genetically modified epidermal sheets were transplanted onto epidermolysis bullosa skin patient (left panel). Follow-up examination of the graft at 8 days and 10 months (middle and right panel) after transplantation. (D) Hematoxylin/eosin staining of grafted skin section at 4 months after transplantation shows a fully differentiated epidermis attached to the dermis (left panel). Immunofluorescence analysis of the grafted skin sections at 4 month (right panel) compared to an unaffected skin section (middle panel). The LAMB3 (green) expression is indistinguishable between the skin of a healthy individual and the genetically corrected skin graft (middle and right panel respectively). Bars scale, 50μm. Reprint by permission from MacMilian publisher; Mavilio et al., Nature Medicine, 2006).